Real Microscope Images & Videos of Induced Pluripotent Stem Cells

Videos: Courtesy of SBI Biosystems in Menlo, CA (video attained with a Nikon BioStation IM):

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Frozen human iPS cells:

Human induced pluripotent stem (iPS) cell colonies created from adult skin cells. Blue represents all DNA. Green and red indicates cell surface proteins found on pluripotent cells.  Photo courtesy of William Collins in the lab of Deepak Srivastava and Christopher Schlieve at the  Gladstone Institutes.

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Human embryonic stem cell colony and iPS colony:

Two colonies of pluripotent stem cells -- one of human embryonic stem cells (left) and one of reprogrammed adult cells (right). This photo was taken in the lab of Jeanne Loring  at  The Scripps Research institute.

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Time-course of reprogramming mouse fibroblasts into induced pluripotent stem cells:

Fibroblasts were transduced with the four reprogramming factors Oct4, Sox2, Klf4 and c-Myc and cultured in mouse embryonic stem cell culture media.  Within 12 days post-transduction the morphology of the fibroblasts changed considerably and by the 12th day distinct iPS colonies readily formed. Image courtesy of Harvard Stem Cell Institute.

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Partially reprogrammed mouse fibroblast expressing GFP:

Partially reprogrammed mouse fibroblasts express green fluorescent protein (GFP) driven by the transgenic Oct4 promoter. The expression of GFP indicates that the fibroblasts are beginning to express pluripotency related genes and thus are on their way to becoming reprogrammed into induced pluripotent cells. Image courtesy of Harvard Stem Cell Institute.